Bayern Munich's Luca Toni, Italy's main striker in 2006 and at Euro 2008, was left out of the initial 30, with Udinese's Antonio Di Natale likely to lead the Azzurri attack.
Rossi, born in New Jersey, was a member of the Parma youth team before moving to Old Trafford in 2005, however he only scored one goal in a spell in English football that included a loan stint at Newcastle.
A successful loan spell at Parma was then followed by a £6.6m move to Villarreal, since when he forced his way into the Italian national team, notably scoring twice against the United States in the 2009 Confederations Cup.
The Italians begin their campaign against Paraguay in Cape Town on 14 June.
Italy squad:
Goalkeepers: Gianluigi Buffon (Juventus), Morgan De Sanctis (Napoli), Federico Marchetti (Cagliari),
Defenders: Salvatore Bocchetti (Genoa), Leonardo Bonucci (Bari), Fabio Cannavaro (Juventus), Giorgio Chiellini (Juventus), Domenico Criscito (Genoa), Christian Maggio (Napoli), Gianluca Zambrotta (AC Milan)
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