Neville has made 80 caps for his country
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England defender Gary Neville has suffered a setback in his hopes of returning to full fitness at the World Cup finals in Germany.
The 31-year-old was hoping to return from a calf problem for the quarter-finals, providing England progress past Ecuador.
On Saturday, England coach Sven-Goran Eriksson said: "He worked yesterday out on the pitch but felt it this morning."
Eriksson added: "So it is one step backwards for him unfortunately."
After a scan on Wednesday, Neville had shown significant improvement but he now looks to be a major doubt for the tournament.
Neville took up his usual right-back position for England's opening 1-0 victory over Paraguay on 10 June.
However, the Manchester United player picked up the problem during training and sat out England's final group games against Trinidad and Tobago and Sweden.