The American crayfish has been discovered in the River Till
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The salmon fishing industry worth £18m a year on the River Tweed is under renewed threat from a major predator.
American signal crayfish have been found in the River Till - a tributary which joins the Tweed near Coldstream.
Nick Yonge of the Tweed Foundation said the discovery of 24 crayfish, which eat young salmon and disturb their natural habitat, was a major blow.
Last year the crayfish were discovered in other tributaries at the Ettrick Water and Leithen Water.